HeLa Nuclear Histone Prep Cell Lysate

Product Discontinued

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01mg per lane.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Triton Extraction Buffer (1 X PBS containing 0.5% Triton X 100 (v/v) and a cocktail of phosphatase and protease inhibitors) with 1 X Lithium Dodecyl Sulfate (LDS) Sample Buffer at pH 8.4. BME has not been added.
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Reproductive
• Subcellular Fraction: Nuclear Histone Prep

Background

HeLa cells are the oldest and most commonly used human cell line in research. HeLa was derived from cervical cancer cells taken on February 9, 1951 from Henrietta Lacks. They were immortalized by George Otto Gey at Johns Hopkins University. HeLa was used by Jonas Salk to test the polio vaccine in the 1950s and have been used extensively in research since then, having been used in over 60,000 publications to date. In March of 2013, the HeLa genome was sequenced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.