Human HAMA ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. HAMA (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human anti-mouse antibody. |
| Standard Curve Range |
3 to 360 ng/mL |
| Sensitivity |
3 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 8.30% |
| Intra-Assay |
CV% < 9.03% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Calibrator diluent, Animal serum with buffer and preservative.
- HAMA Microtiter plate, Pre-coated with mouse IgG
- HAMA Standard, Rabbit anti Mouse IgG antibody (800 ng/vial) in a buffered protein base with Preservative, lyophilized.
- HRP Conjugate, HRP conjugate to Mouse IgG
- Stop solution, 2N Sulphuric Acid (H2SO4). Caution: CausticMaterial!
- Substrate A, Buffered solution with H2O2.
- Substrate B, Buffered solution with TMB.
- Wash Buffer (20x), 20-fold concentrated solution of buffered surfactant.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Publications for HAMA ELISA Kit (KA1010)(3)
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Product General Protocols
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FAQs for HAMA ELISA Kit (KA1010). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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