| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | BTK-94C |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/ml |
| Description | Halotyrosine, clone BTK-94C is recommended for detection of bromotyrosine modified proteins. Halogenation is a chemical reaction that substitutes a molecule’s hydrogen atom with halogen, a nonmetal element. Fluorination, chlorination, bromination and iodination are the four types of halogenation. Halogenated organic compounds are found as natural products in many living organisms. Halotyrosine residues are the result of tyrosine modification, usually bromine or chlorine. This generally occurs as a result of immune cell actions or oxidative stress. For example, activated eosinophils release eosinophil peroxidase, which in turn produces hypobromite (HOBr). Hypobromite can then react with proteins to create bromotyrosine residues. Studies on total bromotyrosine levels have shown that these protein modifications are increased in asthmatics, but are decreased in response to anti-inflammatory drugs. |
| Immunogen | Bromotyrosine structural mimic conjugated to KLH |
| Isotype | IgM |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C. |
| Buffer | PBS containing 0.1% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.1 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")