Halotyrosine Antibody (BTK-94C)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IA, ICC/IF, IP
Clone
BTK-94C
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Halotyrosine Antibody (BTK-94C) Summary

Description
Halotyrosine, clone BTK-94C is recommended for detection of bromotyrosine modified proteins. Halogenation is a chemical reaction that substitutes a molecule’s hydrogen atom with halogen, a nonmetal element. Fluorination, chlorination, bromination and iodination are the four types of halogenation. Halogenated organic compounds are found as natural products in many living organisms. Halotyrosine residues are the result of tyrosine modification, usually bromine or chlorine. This generally occurs as a result of immune cell actions or oxidative stress. For example, activated eosinophils release eosinophil peroxidase, which in turn produces hypobromite (HOBr). Hypobromite can then react with proteins to create bromotyrosine residues. Studies on total bromotyrosine levels have shown that these protein modifications are increased in asthmatics, but are decreased in response to anti-inflammatory drugs.
Immunogen
Bromotyrosine structural mimic conjugated to KLH
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4°C.
Buffer
PBS containing 0.1% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Halotyrosine Products

Array NBP2-62808

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