H/M Pluripotent Stem Cell Flow Cytometry Kit

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Flow Cytometry: H/M Pluripotent Stem Cell Flow Cytometry Kit [FMC001-NOV] - Mouse D3 embryonic stem cells were stained using reagents included in the Human/Mouse Embryonic Stem Cell Multi-Color Flow Cytometry Kit. Cells ...read more
Flow Cytometry: H/M Pluripotent Stem Cell Flow Cytometry Kit [FMC001-NOV] - BG01V human embryonic stem cells were stained using reagents included in the Human/Mouse Pluripotent Stem Cell Multi-Color Flow Cytometry Kit. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow

Order Details

H/M Pluripotent Stem Cell Flow Cytometry Kit Summary

Description
This product is designed for the flow cytometric analysis of human/mouse PSCs using four fluorochrome-conjugated antibodies.

Features
  • Verifies stem cell pluripotency
  • Defines starting population to reduce variability
  • Simultaneously detects four markers of pluripotency
  • Does not require the use of secondary antibodies

Kit Type
Flow Cytometry Kit

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.

Kit Components

Components
  1. Positive Marker: 250 uL of Oct-3/4-APC Rat IgG2B; Clone 240408
  2. Positive Marker: 250 uL of SOX2-PE Mouse IgG2A; Clone 245610
  3. Marker (Positive for human; Negative for mouse): 250 uL of SSEA-4-CFS Mouse IgG3; Clone MC-813-70
  4. Marker (Positive for mouse; Negative for human): 250 uL of SSEA-1-PerCP Mouse IgM; Clone MC-480
  5. 250 uL of Rat IgG2B-APC Isotype Control
  6. 250 uL of Mouse IgG2A-PE Isotype Control
  7. 250 uL of Mouse IgG3-CFS Isotype Control
  8. 250 uL of Mouse IgM-PerCP Isotype Control
  9. 30 mL of 1X Fixation/Permeabilization Buffer
  10. 30 mL of 1X Permeabilization/Wash Buffer

Background

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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