H-2Dk Antibody (15-5-5S) - Azide and BSA Free

Flow Cytometry: H-2Dk Antibody (15-5-5S) [NBP1-28193] - Analysis of C3H/He splenocytes.

• Reactivity: Mu
• Applications: ELISA, Flow, IP, B/N
• Clone: 15-5-5S
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

H-2Dk Antibody (15-5-5S) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse H-2Dk Antibody (15-5-5S) - Azide and BSA Free (NBP1-28193) is a monoclonal antibody validated for use in ELISA, Flow and IP. Anti-H-2Dk Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: C3H mouse splenocytes
• Specificity: An epitope on mouse H-2Dk MHC class I alloantigen
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for H-2Dk Antibody (15-5-5S) - Azide and BSA Free

• H2Dk
• MHC class I

Background

MAb 15-5-5S reacts with the H-2Dk class I alloantigen. It cross-reacts with H-2Kd and with cells from mice with the H-2f haplotype. (1) The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for H-2Dk Antibody (NBP1-28193)(2)

Publications using NBP1-28193

• Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens J Immunol
  
  Details:
  This publication used the FITC conjugated form of this antibody (Cat# NBP1-28194).
• Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 1980-01-31 [PMID: 7188699]
  
  Details:
  Citation using the FITC format of this antibody.