Guinea Pig IgG, Fc Native Protein

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Product Details

Summary
Reactivity GPSpecies Glossary
Applications WB, ELISA, IP
Concentration
1.0 mg/ml

Order Details

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Guinea Pig IgG, Fc Native Protein Summary

Description
Native Guinea Pig IgG, Fc protein.

Source: Guinea Pig Serum

Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum, anti-Guinea Pig IgG and anti-Guinea Pig IgG F(c). No reaction was observed against anti-Guinea Pig IgG F(ab')2 or anti-Papain.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This antibody possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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