Recombinant E. coli GrpE Protein

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SDS-Page: GrpE Protein [NBC1-18370] - GrpE, 21.8kDa (197aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity EcSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant E. coli GrpE Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-197 of E.coli GrpE

Source: E.coli

Amino Acid Sequence: MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
grpE
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBC1-18370.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant E. coli GrpE Protein

  • Heat shock protein B25.3
  • HSP24
  • HSP-70 cofactor

Background

GrpE,co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for GrpE Recombinant Protein (NBC1-18370)(1)


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Other Available Formats

Additional GrpE Products

Array NBC1-18370

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Bioinformatics

Gene Symbol grpE