Recombinant E. coli GrpE Protein

SDS-Page: Recombinant E. coli GrpE Protein [NBC1-18370] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Ec
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant E. coli GrpE Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-197 of E.coli GrpE
  
  

  Source: E.coli

  Amino Acid Sequence: MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: grpE
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant E. coli GrpE Protein

• Heat shock protein B25.3
• HSP24
• HSP-70 cofactor

Background

GrpE ,co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for GrpE Recombinant Protein (NBC1-18370)(1)

Publication using NBC1-18370

• Li J, Gu L, Aach J, Church GM. Improved cell-free RNA and protein synthesis system. PLoS ONE. 2014-09-03 [PMID: 25180701]

Bioinformatics

• Gene Symbol: grpE