GroEL Antibody (9A1/2) - BSA Free

Western Blot: GroEL Antibody (9A1/2) [NBP1-97895] - Analysis of GroEL, mAb (9A1/2): Lane 1: GroEL ( E. coli ) (rec) Protein, Lane 2: HSP60 (Human) (rec) (negative control), Lane 3: E. coli Lysate, Lane 4: HeLa Lysate, heat shocked (negative control)

• Reactivity: Ec
• Applications: WB, IP
• Clone: 9A1/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

GroEL Antibody (9A1/2) - BSA Free Summary

• Immunogen: E. coli GroEL.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: groL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:10000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Recognizes Bacteria GroEL.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for GroEL Antibody (9A1/2) - BSA Free

• Cpn60 chaperonin GroEL, large subunit of GroESL
• ECK4137
• groEL
• JW4103
• mopA

Background

GroEL and GroES are E. coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GroEL Antibody (NBP1-97895)(2)

Publications using NBP1-97895

• Srivastava R, Cha HJ, Peterson MS et al. Antisense downregulation of sigma(32) as a transient metabolic controller in Escherichia coli: effects on yield of active organophosphorus hydrolase. Appl Environ Microbiol. 2000-10-01 [PMID: 11010884]
• Dukan S, Farewell A, Ballesteros M et al. Protein oxidation in response to increased transcriptional or translational errors. Proc Natl Acad Sci U S A. 2000-05-01 [PMID: 10811907]

Bioinformatics

• Gene Symbol: groL