Granulocytes Antibody (HIS48)

• Reactivity: Rt
• Applications: Flow, IHC
• Clone: HIS48
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Granulocytes Antibody (HIS48) Summary

• Description: Novus Biologicals Mouse Granulocytes Antibody (HIS48) (NB100-64933) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PVG rat spleen cell suspension
• Specificity: Recognizes granulocytes and erythroid cells. Clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (3).
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Neat
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20
  • Immunohistochemistry-Paraffin
• Application Notes: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
• Preservative: <0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Granulocytes Antibody (HIS48)

• Basophils
• Eosinophils
• granular leukocytes
• Mast Cells
• Neutrophils
• PML
• PMN
• PMNL
• polymorphonuclear leukocytes

Background

Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Entrez:
  • 295647(Rat)