Granulocytes Antibody (HIS48)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
HIS48
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Granulocytes Antibody (HIS48) Summary

Immunogen
PVG rat spleen cell suspension
Specificity
Recognizes granulocytes and erythroid cells. Clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (3).
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CSF3
Purity
Tissue culture supernatant
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry Neat
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2M Tris/HCl (pH 7.4) and 8% fetal Bovine serum (FBS)
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Granulocytes Antibody (HIS48)

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3OS
  • Filgrastim
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • pluripoietin

Background

Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Granulocytes NB100-64933

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Bioinformatics

Gene Symbol CSF3
Entrez