Granulocytes Antibody (HIS48) Summary
PVG rat spleen cell suspension
Recognizes granulocytes and erythroid cells. Clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (3).
Tissue culture supernatant
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- Flow Cytometry Neat
- Immunocytochemistry/Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:20
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.2M Tris/HCl (pH 7.4) and 8% fetal Bovine serum (FBS)
0.09% Sodium Azide
Tissue culture supernatant
Alternate Names for Granulocytes Antibody (HIS48)
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
- granulocyte colony-stimulating factor
Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells.
Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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