![Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - IHC-Prediluted [NBP2-48057] - Formalin-fixed, paraffin-embedded tonsil stained with Granulocyte MAb (SPM250). Note specific cytoplasmic staining.](http://images.novusbio.com/fullsize/Granulocyte-Marker-Antibody-SPM250-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48057-img0001.jpg "Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - IHC-Prediluted [NBP2-48057] - Formalin-fixed, paraffin-embedded tonsil stained with Granulocyte MAb (SPM250). Note specific cytoplasmic staining.")
| Reactivity | Hu, RMSpecies Glossary |
| Applications | IHC |
| Clone | SPM250 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes. |
| Localization | Cytoplasmic |
| Marker | Macrophage / Granulocyte Marker |
| Specificity | Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. monoclonal antibody BM-2 reacts with early precursor and mature forms of human myeloid cells. This monoclonal antibody is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")