![Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - Azide and BSA Free [NBP2-34750] - Formalin-fixed, paraffin-embedded tonsil stained with Granulocyte MAb (SPM250). Note specific cytoplasmic staining.](http://images.novusbio.com/fullsize/Granulocyte-Marker-Antibody-SPM250-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34750-img0001.jpg "Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - Azide and BSA Free [NBP2-34750] - Formalin-fixed, paraffin-embedded tonsil stained with Granulocyte MAb (SPM250). Note specific cytoplasmic staining.")
| Reactivity | Hu, RMSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | SPM250 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32856). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes. |
| Localization | Cytoplasmic |
| Marker | Macrophage / Granulocyte Marker |
| Specificity | Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. monoclonal antibody BM-2 reacts with early precursor and mature forms of human myeloid cells. This monoclonal antibody is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA: For coating, order Ab without BSA. Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")