![Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813] - Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).](http://images.novusbio.com/fullsize/Golgi-Complex-Antibody-SPM581-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34813-img0001.jpg "Immunohistochemistry-Paraffin: Golgi Complex Antibody (SPM581) - Azide and BSA Free [NBP2-34813] - Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SPM581 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-32853). Antibody with azide - store at 2 to 8C. |
| Immunogen | SU-DHL-1 large cell lymphoma cells. |
| Localization | Golgi complex in cytoplasm |
| Marker | Marker for Human Cells |
| Specificity | This monoclonal antibody recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunocytochemistry (Acetone or paraformaldehyde fixed): 1-2ug/ml for 30 min. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")