Glucoamylase Antibody

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Western Blot: Glucoamylase Antibody [NBP1-78076] - Goat anti Glucoamylase antibody was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target ...read more

Product Details

Summary
Reactivity FuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Glucoamylase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Glucoamylase [Rhizopus]. Uniprot ID P07683
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:3000
  • ELISA 1:5000 - 1:25000
Application Notes
This product has been assayed against 1.0 ug of Glucoamylase [Rhizopus] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:5,000 - 1:25,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reactive in Rhizopus

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 100 ul deionized water.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-78076

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