Glu-Glu Epitope Tag Antibody

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Western Blot: Glu-Glu Epitope Tag Antibody [NB600-353] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-Glu-Glu antibody used for ...read more
Western Blot: Glu-Glu Epitope Tag Antibody [NB600-353] - Analysis using the HRP conjugate of NB600-353. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used ...read more

Product Details

Summary
Reactivity Ec, NASpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Glu-Glu Epitope Tag Antibody Summary

Immunogen
Goats were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polymavirus conjugated to KLH.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:30000
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glu-Glu Epitope Tag Antibody

  • EYMPME epitope tag
  • EYMPME tag
  • Glu-Glu epitope tag

Background

The Glu-Glu epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized Glu-Glu epitope represents amino acid residues 314-319, EYMPME, of middle T antigen of mouse polyomavirus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Glu-Glu Epitope Tag Products

Glu-Glu Epitope Tag NB600-353

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