Gliadin peptide Antibody (14D5) - BSA Free

Western Blot: Gliadin peptide Antibody (14D5) - BSA Free [NBP2-23580] - Analysis of Gliadin peptide measured in rye (lane 1), barley (lane 2) and wheat (lane 3). The SDS gel was run under reducing conditions.

Product Discontinued

Gliadin peptide Antibody (14D5) - BSA Free Summary

• Immunogen: Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) adsorbed onto aluminum hydroxide gel
• Epitope: The epitope is located in a central region of the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide.
• Specificity: Clone 14D5 reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide KLQPFPQPQLPYPQPQ.
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 14D5 reacts with both the deamidated peptide (KLQPFPQPELPYPQPQ) and non-deamidated petide (KLQPFPQPQLPYPQPQ) when these are coated directly in an immunoplate, but approx. 20-fold higher concentrations of antibody are needed to produce the same signal with the non-deamidated peptide. Sandwhich ELISA is not recommended for this product, please use direct ELISA.

Reactivity Notes

Wheat

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Background

Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.