Gemtuzumab Antibody (975155) [Unconjugated]

Western blot shows Rituximab (negative control), Trastuzumab (negative control), Gemtuzumab, and Brentuximab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody HAF008). A specific band was detected for Gemtuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

U937 Human histiocytic lymphoma cell line was stained with anti-Siglec-3/CD33 (Gemtuzumab) (MAB9635), then Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171, filled histogram) or Mouse IgG2AIsotype Control Antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).

Biotinylated biosimilars were serially diluted and incubated with Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB96171, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.

A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB96171, 333 ng/mL) detected Gemtuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.

The anti-Idiotype antibody (catalog number MAB96171) directed against Gemtuzumab was coated at 4 μg/mL in a 96-well polystyrene plate to capture the biosimilar Gemtuzumab. Biotinylated Anti-Gemtuzumab used at0.5 μg/mLwas used as the detection antibody before incubation with Streptavidin-HRP. Other biosimilars did not bridge the anti-idotype antibody, showing specificity of the anti-Gemtuzumab (anti-Idiotype) antibody.

• Reactivity: All-Multi
• Applications: WB, ELISA, Flow
• Clone: 975155
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Gemtuzumab Antibody (975155) [Unconjugated] Summary

• Immunogen: Full length Gemtuzumab
• Specificity: Detects Gemtuzumab in Western blots and direct ELISAs. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gemtuzumab Antibody (975155) [Unconjugated]

• Gemtuzumab

Background

The anti-Gemtuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Gemtuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.