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Non-species specific GABA - Ready-To-Use ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
31.2 - 2000 pg/mL (example only; lot dependent)
Sensitivity
10.9 pg/mL (example only; lot dependent)

Order Details

Non-species specific GABA - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
31.2 - 2000 pg/mL (example only; lot dependent)
Sensitivity
10.9 pg/mL (example only; lot dependent)
Assay Type
Competitive ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Alternate Names for Non-species specific GABA - Ready-To-Use ELISA Kit (Colorimetric)

  • y-Aminobutyric acid

Background

Without colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GABA ELISA Kit (NBP3-55598). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Additional GABA Products

Blogs on GABA.

Brain size matters: MTOR regulates autophagy and number of cortical interneurons
By Jamshed Arslan Pharm.D. Interneurons transmit impulses between other neurons, in part, to facilitate the birth of neurons. Cortical interneurons themselves arise from the progenitors in the ventral telencephalo...  Read full blog post.

Using RPE65 as a tool to investigate ocular gene therapies
While not life threatening, blindness and retinal disease are profoundly debilitating and greatly affect quality of life.  Understandably, gene therapy has been subject to controversy given it’s potential effects on the rest of our cellular ...  Read full blog post.

New MECP2 Study Suggests Important Link to GABA
MECP2 antibodies are used in DNA methylation studies as well as research into Rett syndrome, a progressive neurological disorder caused by a mutation in the MECP2 gene. Now, a new study has been published by the laboratory where the Rett Syndrome gene...  Read full blog post.

PMP22 Antibodies Assist in PNS Myelin Repair Research
Novus Biologicals recently added a new  PMP22 antibody, conjugated to Biotin, to our antibody database. PMP22 (Peripheral Myelin Protein 22) is important to the structure of the myelin sheath in peripheral nerves, and is encoded by the PMP22 gene.Th...  Read full blog post.

A Shocking Truth: GABA Antibodies Developing New Therapies for Clinical Depression
Supplying antibodies for neuroscience research forms an important part of our work here at Novus Biologicals. Our antibody database is represented by more than 5,600 neuroscience products covering conditions ranging from sensory systems to neurodegene...  Read full blog post.

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