Sheep FSH ELISA Kit (Colorimetric)

ELISA: Sheep FSH ELISA Kit (Colorimetric) [NBP2-60023] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Sh
• Applications: ELISA
• Suitable Sample Type: serum, plasma, tissue homogenates
• Standard Curve Range: 2 mIU/ml -70 mIU/ml
• Sensitivity: 1 mIU/ml

Sheep FSH ELISA Kit (Colorimetric) Summary

• Specificity: This assay has high sensitivity and excellent specificity for detection of sheep FSH. No significant cross-reactivity or interference between sheep FSH and analogues was observed.
• Standard Curve Range: 2 mIU/ml -70 mIU/ml
• Sensitivity: 1 mIU/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 15%
• Intra-Assay: CV% < 15%
• Sample Volume: 50-100ul
• Kit Type: ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for FSH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.