FoxP3 Fixation & Permeabilization Flow Cytometry Kit

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Product Details

Summary
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FoxP3 Fixation & Permeabilization Flow Cytometry Kit Summary

Description
FlowXTM FoxP3 Fixation & Permeabilization Buffer Kit is formulated and optimized for immunofluorescent staining of FoxP3+ single-cell suspensions intended for flow cytometric acquisition and analysis. This product is supplemented with the metabolic inhibitor sodium azide.

This product is designed for flow cytometry applications with fluorochrome-conjugated antibodies. Refer to product specification sheets to obtain the recommended working antibody dilutions.

Kit Type
Flow Cytometry Kit
Gene
FOXP3

Applications/Dilutions

Dilutions
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. FoxP3 Fixation/ Transcription Factor Diluent
  2. FoxP3 Fixation/Transcription Factor Concentrate (4X)
  3. FoxP3/ Transcription Factor
  4. Permeabilization and Wash Buffer (10X)

Background

Human FOXP3 is a 47 kDa member of the P subclass of the forkhead box family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (forkhead) DNA-binding domain. In human, FOXP3 is found in CD4+, CD8+ and CD4+CD25+ T cells and directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FOXP3