Mouse Folic acid ELISA Kit (Colorimetric)

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ELISA: Mouse Folic acid ELISA Kit (Colorimetric) [NBP2-59996] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
0.195 ng/ml-50 ng/ml
Sensitivity
0.195 ng/ml

Order Details

Mouse Folic acid ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of mouse FA. No significant cross-reactivity or interference between mouse FA and analogues was observed.
Standard Curve Range
0.195 ng/ml-50 ng/ml
Sensitivity
0.195 ng/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Stop Solution
  2. Standard (Freeze dried)
  3. Sample Diluent
  4. TMB Substrate
  5. Wash Buffer (25 x concentrate)
  6. Antibody (100 x concentrate)
  7. Antibody Diluent
  8. Assay plate
  9. Adhesive Strip (For 96 wells)
  10. HRP-conjugate Diluent
  11. HRP-conjugate (100 x concentrate)
  12. Instruction manual

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Mouse Folic acid ELISA Kit (Colorimetric)

  • 5 Methyl THFA
  • 5 Methyltetrahydrofolate
  • Folate
  • Tetrahydrofolate
  • Tetrahydrofolic acid
  • THFA

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for FA and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated FA and FA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of FA in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Folic acid ELISA Kit (NBP2-59996). (Showing 1 - 2 of 2 FAQ).

  1. We received an inquiry about Folic acid ELISA Kit, Cat# NBP2-59996. The customer would like to know the plate type in this kit. Could you tell us whether the assay plate is strip type (8×12 well) or one strip 96-well type?
    • These are 96 well plates and yes, the plate can be divided into the 8x12 strips.
  2. We want to know the plate type in your Folic acid ELISA Kit (NBP2-59996). Could you tell us whether the assay plate is strip type (8×12 well) or  one strip 96-well type?
    • The plate of this product can be divided into the 8x12 strips.

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