Fluorescein Antibody

Images

 
Western Blot: Fluorescein Antibody [NB600-493] - Lane 1: BSA conjugated Fluoresccein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein primary antibody at 1:1,000 overnight at 4C. Secondary antibody: ...read more
Western Blot: Fluorescein Antibody [NB600-493] - Analysis using the Biotin conjugate of NB600-493. Detection of Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1000 for 60 ...read more
Western Blot of Fluorescein Antibody. Lane 1: Opal Prestained Molecular Weight Marker

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

View Available Conjugates

Fluorescein Antibody Summary

Description
Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Store vial at 4C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
Fluorescein conjugated to Goat IgG
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:25000
  • Flow Cytometry
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:250-1:1500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:500-1:3000
Application Notes
This product has been tested by ELISA and western blot and is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Use in IHC-FrFl reported in scientific literature (PMID:35411060)

Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 30228102).
Publications
Read Publications using
NB600-493 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fluorescein Antibody

  • FITC
  • fluorescein isothyocianate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fluorescein Antibody (NB600-493)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IF/IHC, IHC-Fr, IHC-FrFl, IHC-WhMt.


Filter By Application
IF/IHC
(5)
IHC-Fr
(2)
IHC-FrFl
(1)
IHC-WhMt
(1)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB600-493 Applications Species
Shah S, Ulm J, Sifri ZC et al. Mobilization of bone marrow cells to the site of injury is necessary for wound healing J Trauma 2009-08-01 [PMID: 19667884] (IHC-Fr)

Details:
This citation used the Texas Red version of this antibody.
IHC-Fr
Shah S, Ulm J, Sifri ZC et al. Mobilization of bone marrow cells to the site of injury is necessary for wound healing J Trauma (IHC-Fr)

Details:
This publication used the Texas Red conjugated form of this antibody (Cat# NB110-3648).
IHC-Fr
Magrinelli E, Baumann N, Wagener RJ et al. Heterogeneous fates of simultaneously-born neurons in the cortical ventricular zone Scientific reports 2022-04-11 [PMID: 35411060] (IHC-FrFl) IHC-FrFl
Deryckere A, Styfhals R, Elagoz AM et al. Identification of neural progenitor cells and their progeny reveals long distance migration in the developing octopus brain eLife 2021-08-24 [PMID: 34425939]
Vieceli FM, Bronner ME. Leukocyte Receptor Tyrosine Kinase interacts with secreted midkine to promote survival of migrating neural crest cells. Development 2018-09-18 [PMID: 30228102] (IHC-WhMt) IHC-WhMt
Magrinelli E, Wagener R, Jabaudon D. Simultaneous production of diverse neuronal subtypes during early corticogenesis. bioRxiv 2018-07-16 (IF/IHC, Mouse) IF/IHC Mouse
Hutchins EJ, Bronner ME. Draxin alters laminin organization during basement membrane remodeling to control cranial neural crest EMT Dev Biol 2018-12-24 [PMID: 30579765] (IF/IHC) IF/IHC
Piacentino M. L. , Bronner M. E. Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction. PLoS Biol 2018-06-01 [PMID: 29949573] (IF/IHC, IF/IHC) IF/IHC, IF/IHC
Uribe RA, Buzzi AL, Bronner ME et al. Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression J Cell Biol 2015-11-25 [PMID: 26598618] (IF/IHC) IF/IHC

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Product General Protocols

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Secondary Antibodies

 

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Array NB600-493

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