FliC Antibody (FLIC-1)

Product Discontinued

FliC Antibody (FLIC-1) Summary

• Immunogen: The immunogen was the full length version of the protein: FliC (FLIC-1)
• Specificity: This antibody recognizes the 51-44kd flagellin subunit component FliC (FLIC-1).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Flagellar subunit protein FliC expressed in various bacteria including Salmonella, Escherichia coli, and others.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for FliC Antibody (FLIC-1)

• Flagellin component FliC

Background

The FLIC-1 monoclonal antibody recognizes the flagellin subunit component FliC. FliC is a 51-44 kD protein expressed in many motile enteric bacteria including Salmonella and Escherichia. FliC is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers become polymerized to form the tail filament. The C-terminus of FliC binds to the export chaperone FliS. This interaction is thought to facilitate FliC polymerization and prevent FliC from polymerizing prematurely in cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FliC Antibody (NB100-78498)(1)

Publication using NB100-78498

• Zervos, C;Zimmerman, TP;Willis, T;Flexman, G;Srivastava, J;Silverstein, R;Williams, M;Vandeberg, P;Culp, JL;Burns, D;Barham, V;Durham, A;Malinzak, DA; Immunoglobulin G from single plasma donor in immune globulin intravenous causes false positive pyrogen test Biologicals 2019-04-22 [PMID: 31023510] (In vitro, Rabbit)