Recombinant Flagellin His (C-Term) Protein

• Applications: Func, PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Flagellin His (C-Term) Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 503 amino acids corresponding to Flagellin , with Leu, Glu and 6x His at C-terminus Source: E. coli

  Uniprot ID: P06179

  Amino Acid Sequence: MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH
  
  A single non-glycosylated polypeptide chain containing 503 amino acids corresponding to Flagellin , with Leu, Glu and 6x His at C-terminus Source: E. coli

  Uniprot ID: P06179

  Amino Acid Sequence: MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Flagellin as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • In vitro assay
  • SDS-Page
• Application Notes: Use in In vitro assay reported in scientific literature (PMID:35021065)
• Theoretical MW: 52.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Background

Flagellin arranges itself in a hollow cylinder to form the filament in bacterial flagellum. It is the principal substituent of bacterial flagellum, and is present in large amounts on nearly all flagellated bacteria. Mammals often have acquired immune responses (Tcell and antibody responses) to flagellated bacterium and the plant defense mechanisms can be activated by the conserved Nterminal part of flagellin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Flagellin Recombinant Protein (NBP2-35885)(3)

Publications using NBP2-35885

• Rana N, Privitera G, Kondolf HC et al. GSDMB is increased in IBD and regulates epithelial restitution/repair independent of pyroptosis Cell 2022-01-20 [PMID: 35021065] (In vitro)
• Campden R, Warren A, Greene C Et al. Extracellular cathepsin Z signals through the alpha 5 integrin and augments NLRP3 inflammasome activation Journal of Biological Chemistry 2021-12-01 [PMID: 34864055]
• Duckworth CA, Campbell BJ, Poroikov V Using systems medicine to identify a therapeutic agent with potential for repurposing in Inflammatory Bowel Disease Dis Model Mech 2020-09-22 [PMID: 32958515]