Recombinant Flagellin Protein

Images

 
There are currently no images for Flagellin Recombinant Protein (NBP2-35885).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Applications PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations

Recombinant Flagellin Protein Summary

Description
A biologically active protein to Flagellin with a C-terminal His-Tag.

Source: E. coli

Amino Acid Sequence: MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAIANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSANSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGANDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDNSTFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSVDKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAGVTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKYTADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEAAATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSARSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH
Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of rFlagellin, His as determined by LAL method.

Applications/Dilutions

Theoretical MW
52.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-35885.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Background

Flagellin arranges itself in a hollow cylinder to form the filament in bacterial flagellum. It is the principal substituent of bacterial flagellum, and is present in large amounts on nearly all flagellated bacteria. Mammals often have acquired immune responses (Tcell and antibody responses) to flagellated bacterium and the plant defense mechanisms can be activated by the conserved Nterminal part of flagellin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Flagellin Recombinant Protein (NBP2-35885)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-35885 Applications Species
Duckworth CA, Campbell BJ, Poroikov V Using systems medicine to identify a therapeutic agent with potential for repurposing in Inflammatory Bowel Disease biorxiv.org Jan 1 2019

Reviews for Flagellin Recombinant Protein (NBP2-35885) (0)

There are no reviews for Flagellin Recombinant Protein (NBP2-35885). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Flagellin Recombinant Protein (NBP2-35885) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Flagellin Products

Blogs on Flagellin.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Flagellin Protein and receive a gift card or discount.