FKBP12/FKBP12.6 Antibody (426227) [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat FKBP12/FKBP12.6 Monoclonal Antibody (Catalog # MAB4174) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP12 and FKBP12.6 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows recombinant human FKBP 12, recombinant human FKBP 12.6, recombinant human FKBP 13, recombinant human FKBP 25, recombinant human FKBP 38, and recombinant human FKBP 52. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat FKBP12/FKBP12.6 Monoclonal Antibody (Catalog # MAB4174) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP12 and FKBP12.6 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FKBP12 and FKBP12.6 was detected in immersion fixed paraffin-embedded sections of human brain (caudate putamen) using Rat Anti-Human/Mouse/Rat FKBP12/FKBP12.6 Monoclonal Antibody (Catalog # MAB4174) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 426227
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

FKBP12/FKBP12.6 Antibody (426227) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FKBP12.6
  Gly2-Glu108
  Accession # P68106
• Specificity: Detects human FKBP12.6 in direct ELISAs. Detects human, mouse, and rat FKBP12 and FKBP12.6 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FKBP12/FKBP12.6 Antibody (426227) [Unconjugated]

• FKBP12/FKBP12.6

Background

FK506 binding protein, 12.6 kDa molecular weight (FKBP12.6), also called FKBP1B, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑ proline residues critical for proper folding of proteins. The immunosuppressant FK506 is a potent inhibitor of FKBP12.6. FKBP12.6 is localized in the sarcoplasmic reticulum associated with the Ryanodyne receptor RYR2. PKA-induced phosphorylation of RYR2 causes FKBP12.6 dissociation. In heart failure, RYR2 is hyperphosphorylated and has low levels of FKBP12.6 bound to it. Male FKBP12.6 knockout mice have cardiac hypertrophy and lethal exercise arrhythmias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P68106()