Fixation and Permeabilization Flow Cytometry Kit Summary
The Fixation and Permeabilization Flow Cytometry Kit is suitable for the analysis of normal and malignant leukocyte populations derived from various human biological samples (blood, bone marrow and others) using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation.The kit contains 2 reagents: Fixation Medium (Reagent A) and Permeabilization Medium (Reagent B). It is intended for first fixing cells in suspension with Reagent A and then permeabilizing the cell membranes with Reagent B. This procedure gives antibodies access to intracellular structures and leaves the morphological scatter characteristics of cells intact. Specific formulations reduce background staining and allow simultaneous addition of permeabilization medium and fluorochrome labeled antibodies.
Flow Cytometry Kit
Packaging, Storage & Formulations
Store at room temperature.
- Reagent A: Fixation Medium (1 x 5ml vial)
- Reagent B: Permeabilization Medium (1 x 5ml vial)
Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc.
Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the Fixation and Permeabilization Flow Cytometry Kit, flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the Fixation and Permeabilization Flow Cytometry Kit; some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
Publications for Fixation and Permeabilization Kit (NBP2-31377) (0)
There are no publications for Fixation and Permeabilization Kit (NBP2-31377).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Fixation and Permeabilization Kit (NBP2-31377) (0)
There are no reviews for Fixation and Permeabilization Kit (NBP2-31377).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
View specific protocols for Fixation and Permeabilization Kit (NBP2-31377):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Fixation and Permeabilization Kit (NBP2-31377) (0)
Other Available Formats
Additional Fixation and Permeabilization Products
Blogs on Fixation and Permeabilization