Fixation and Permeabilization Flow Cytometry Kit

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Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Double labeling of a normal blood sample treated with NBP2-31377, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Flow cytrometric analysis of normal bone marrow (BM) after fixation and pemeabilization using NBP2-31377, followed by immunostaining for ...read more
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Flow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using NBP2-31377, followed by ...read more
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Immunostaining with CD22-PE conjugate of undifferentiated leukemia cells of B-ALL type.
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Immunostaining with Nordic-MUbio CD3-PE conjugate of surface CD3-negative undifferentiated leukemia cells of T-ALL type.
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Immunostaining with Nordic-MUbio anti MPO-FITC conjugate of undifferentiated leukemia cells of AML type.
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Forward and side scatter pattern of a normal blood sample treated with NBP2-31377.
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Double labeling of a normal blood sample treated with NBP2-31377, and immunostained for Lactoferrin (PE) and MPO-C2 (FITC).
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Double labeling of a normal blood sample treated with NBP2-31377, and immunostained for CD3 (PE) and MPO-C2 (FITC).

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Fixation and Permeabilization Flow Cytometry Kit Summary

Description
The Fixation and Permeabilization Flow Cytometry Kit is suitable for the analysis of normal and malignant leukocyte populations derived from various human biological samples (blood, bone marrow and others) using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation.The kit contains 2 reagents: Fixation Medium (Reagent A) and Permeabilization Medium (Reagent B). It is intended for first fixing cells in suspension with Reagent A and then permeabilizing the cell membranes with Reagent B. This procedure gives antibodies access to intracellular structures and leaves the morphological scatter characteristics of cells intact. Specific formulations reduce background staining and allow simultaneous addition of permeabilization medium and fluorochrome labeled antibodies.
Kit Type
Flow Cytometry Kit

Packaging, Storage & Formulations

Storage
Store at room temperature.

Kit Components

Components
  1. Reagent A: Fixation Medium (1 x 5ml vial)
  2. Reagent B: Permeabilization Medium (1 x 5ml vial)

Notes

Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc.

Background

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the Fixation and Permeabilization Flow Cytometry Kit, flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the Fixation and Permeabilization Flow Cytometry Kit; some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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