FITC Antibody - BSA Free

• Reactivity: Rt, All-NA
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FITC Antibody - BSA Free Summary

• Immunogen: FITC conjugated to KLH.
• Specificity: This reacts specifically with FITC. There is no reaction with KLH.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:2000
  • Immunohistochemistry 1:200-1:2000
  • Immunohistochemistry-Paraffin 1:200- 1:2000
  • Western Blot 1:1000 - 1:30000
• Application Notes: Elisa-for coating plates 1:100 - 1:500.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32940130)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FITC Antibody - BSA Free

• FITC
• Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB600-1273)(3)

Publications using NB600-1273

• Bhat MA, Esmaeili A, Neumann E et al. Targeting the Interaction of GABA(B) Receptors With CHOP After an Ischemic Insult Restores Receptor Expression and Inhibits Progressive Neuronal Death Frontiers in Pharmacology 2022-03-29 [PMID: 35422706]
• Bennett M, Chin A, Lee HJ et al. Proteoglycan 4 reduces neuroinflammation and protects the blood-brain barrier after traumatic brain injury J. Neurotrauma 2020-09-17 [PMID: 32940130] (ELISA, Rat)
• Torre ER, Coleman S, Yi H et al. A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain. J Neurosci Methods 2003-05-01 [PMID: 12763227]