FITC Antibody [Unconjugated]

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Product Details

Summary
Reactivity All-MultiSpecies Glossary
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

FITC Antibody [Unconjugated] Summary

Immunogen
FITC-KLH (Fluorescein-isothiocyanate-coupled Keyhole Limpet Hemocyanin)
Specificity
Detects Fluorescein Isothiocyanate (FITC) in direct ELISAs. In direct ELISAs, nocross-reactivity with Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647,Alexa Fluor® 700, Alexa Fluor® 750, NorthernLights™ NL493, NorthernLights™ 557,and NorthernLights™ 637 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Publications
Read Publication using
G-148-C in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and Sodium Azide with Trehalose.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FITC Antibody [Unconjugated]

  • FITC
  • Fluorescein Isothiocyanate

Background

Fluorescein isothiocyante (FITC) is a derivative of fluorescein modified with an isothiocyanate reactive group (-N=C=S).  FITC is often used to label antibodies for immunofluorescent cytochemistry and histochemistry. FITC can be also conjugated to proteins and RNA and DNA probes because such conjugation does not impair biological activity of these molecules. In some experimental setups FITC fluorescence may need to be enhanced, which is done by employing anti-FITC antibodies conjugated either to FITC or other fluorophores with similar excitation and emission spectra.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (G-148-C)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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Mouse
(1)
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