FITC Antibody (SPM395)

• Reactivity: All-NA
• Applications: WB, Flow, Func, ICC/IF, IHC
• Clone: SPM395
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

FITC Antibody (SPM395) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08492)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Fluorescein isothiocyanate (FITC) conjugated to KLH
• Localization: Depends on the target
• Specificity: This monoclonal antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this monoclonal antibody is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • In-situ Hybridization
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Pretreatment depends upon the fluoreceinated primary antibody.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for FITC Antibody (SPM395)

• FITC
• Fluorescein Isothiocyanate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NBP3-07778)(1)

Publication using NBP3-07778

• Zhang AQ, Hostetler A, Chen LE et al. Universal redirection of CAR T cells against solid tumours via membrane-inserted ligands for the CAR Nature biomedical engineering 2023-06-08 [PMID: 37291434] (Immunohistochemistry)