Feline IgG, Fab2 Native Protein

SDS-Page: Feline IgG, Fab2 Native Protein [NBP1-97027] - SDS-PAGE Results of Feline F(ab)2 fragment. Lane 1: Feline F(ab)2 Reduced 10ug.Lane 2: Feline F(ab)2 Reduced 5ug. Lane 3: Feline F(ab)2 Reduced 1ug. Lane 4: Opal Prestained Molecular Weight Marker. Lane 5: Feline F(ab)2 Non-Reduced 10ug. Lane 6: Feline F(ab)2 Non-Reduced 5ug. Lane 7: Feline F(ab)2 Non-Reduced 1ug. 4-20% Gel, Coomassie stained.

Product Discontinued

Feline IgG, Fab2 Native Protein Summary

• Description: Native Feline Feline IgG, Fab2
  
  Source: Normal serum (Accession #:38201661)
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Cat IgG, anti-Cat IgG F(ab')2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin.
• Protein/Peptide Type: Native Protein
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: This product can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.