FAM119A Antibody (OTI2G7) - Azide and BSA Free

Immunohistochemistry: FAM119A Antibody (OTI2G7) - Azide and BSA Free [NBP2-72423] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FAM119A mouse monoclonal antibody.

Flow Cytometry: FAM119A Antibody (OTI2G7) - Azide and BSA Free [NBP2-72423] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FAM119A antibody, and then analyzed by flow cytometry.

Immunohistochemistry: FAM119A Antibody (OTI2G7) - Azide and BSA Free [NBP2-72423] - Staining of paraffin-embedded Human pancreas tissue using anti-FAM119A mouse monoclonal antibody.

Flow Cytometry: FAM119A Antibody (OTI2G7) - Azide and BSA Free [NBP2-72423] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM119A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM119A.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI2G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FAM119A Antibody (OTI2G7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse FAM119A Antibody (OTI2G7) - Azide and BSA Free (NBP2-01334) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human FAM119A(NP_660323) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: METTL21A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 24.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for FAM119A Antibody (OTI2G7) - Azide and BSA Free

• EC 2.1.1.-
• FAM119A
• HCA557B
• Hepatocellular carcinoma-associated antigen 557b
• hypothetical protein LOC151194
• LOC151194
• member A
• methyltransferase like 21A
• MGC45373

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METTL21A