Human Estrone ELISA Kit (Colorimetric) Summary
| Description |
Estrone ELISA Kit is an enzyme immunoassay for the quantitative measurement of estrone. |
| Standard Curve Range |
8.1 to 2400 pg/mL |
| Sensitivity |
14.6 pg/mL |
| Assay Type |
Quantification |
| Inter-Assay |
CV% < 9.5% |
| Intra-Assay |
CV% < 7.67% |
| Spike Recovery |
99.47% |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
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Components
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- Assay Buffer: Ready to use. One vial containing a protein-based buffer with anon-mercury preservative.
- Avidin-Horse Radish Peroxidase (HRP) Conjugate Concentrate (100x):Avidin-HRP conjugate in a protein-based buffer with a non-mercury preservative.
- Estrone-Biotin Antibody Concentrate (100x): Estrone-Biotin Antibody in aprotein-based buffer with a non-mercury preservative.
- Rabbit Anti-Estrone Antibody Coated Microwell Plate: Break Apart Wells. Readyto use. One 96 well polyclonal antibody-coated microwell plate in a resealablepouch with desiccant.
- Stopping Solution: Ready to use. One vial containing 1 M sulfuric acid.
- TMB Substrate: Ready to use. One bottle containing tetramethylbenzidine andhydrogen peroxide in a non-DMF or DMSO containing buffer.
- Wash Buffer Concentrate (10x): One bottle containing buffer with a non-ionicdetergent and a non-mercury preservative.
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Estrone ELISA Kit (Colorimetric)
Background
Estrone, estradiol, and estriol are all estrogens natural to the human body. They belong to the group of steroid hormones, and are mostly responsible for the growth of female characteristics in puberty and also regulation of the menstrual cycle. They are produced primarily in the ovaries but are also manufactured from androstenedione in fat cells, muscle cells, and skin. Oral estrogen is converted to estrone(less potent) by the gut and liver, reversing the normal premenopausal 2:1 ratio of 17b-oestradiol to estrone. Estrone Levels 1. Men 2.90 - 7.50 pg/0.1 ml 2. Women (days of menstrual cycle) 1-10 days 4.30 - 18.0 pg/0.1 ml 11-20 days 7.50 - 19.6 pg/0.1 ml 21-30 days 13.1 - 20.1 pg/0.1 ml
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Publications for Estrone ELISA Kit (KA1908)(4)
Showing Publications 1 -
4 of 4.
| Publications using KA1908 |
Applications |
Species |
| Xiaoya J, Na L, Mei M et al. Comparison of the mechanisms of estrogen disrupting effects between triphenyl phosphate (TPhP) and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP). Ecotoxicol Environ Saf. 2021-12-08 [PMID: 34890987] |
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| Jing L, Hong Y, Lijuan H et al. SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS. Genes Dis. 2019-09-17 [PMID: 33569518] |
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| Zhang K, Yang Q, Yang L et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest. 2019-05-06 [PMID: 31063987] |
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| Thibeault AA, Deroy K, Vaillancourt C, Sanderson JT. A Unique Co-culture Model for Fundamental and Applied Studies of Human Fetoplacental Steroidogenesis and Interference by Environmental Chemicals. Environ Health Perspect. 2014-01-29 [PMID: 24486430] |
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Product General Protocols
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FAQs for Estrone ELISA Kit (KA1908). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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