Epstein Barr Virus Nuclear Antigen 3A Antibody

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Western Blot: Epstein Barr Virus Nuclear Antigen 3A Antibody [NBP2-66589] - Analysis using EBNA-3A antibody on cell lines infected with Epstein Barr virus.

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

Order Details

Epstein Barr Virus Nuclear Antigen 3A Antibody Summary

Immunogen
Recombinant EBNA-3A protein
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 0.25-1 ug/ml
Theoretical MW
102.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.08% Sodium Azide
Purity
Ammonium sulfate precipitation

Background

EBNA-3A is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA-3A functions as a transcriptional regulator though the target genes are currently unknown. Plays an essential role for activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA3A probably contributes to a negative autoregulatory control loop.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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