Epstein Barr Virus Nuclear Antigen 3A Antibody

Western Blot: Epstein Barr Virus Nuclear Antigen 3A Antibody [NBP2-66589] - Analysis using EBNA-3A antibody on cell lines infected with Epstein Barr virus.

• Reactivity: V-Vi
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Epstein Barr Virus Nuclear Antigen 3A Antibody Summary

• Immunogen: Recombinant EBNA-3A protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25-1 ug/ml
• Application Notes: .
• Theoretical MW: 102.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Delipidated, heat inactivated, lyophilized stable whole serum
• Preservative: No Preservative
• Purity: Whole Antiserum

Background

EBNA-3A is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA-3A functions as a transcriptional regulator though the target genes are currently unknown. Plays an essential role for activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA3A probably contributes to a negative autoregulatory control loop.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.