| Immunogen | The antibody reacts with the Eosinophil Major Basic Protein antigen. |
| Specificity | BMK-13 binds to the 10 kD eosinophil Major Basic Protein (MBP) of both resting and activated eosinophils in cytospins and frozen sections of bronchial and skin biopsies of allergic sites and normal sites and thus can be used as a "pan-eosinophil" marker. The antibody BMK-13 stains in frozen sections of bronchial biopsies from atopic asthmatics, rhinitics and normal non-atopic subjects, substantially higher counts of positive cells when compared to EG1, EG2 and chromotrope 2R. BMK-13 cross-reacts weakly with human basophils, which also contain low level of this protein. It does not cross-react with any other human protein or cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This mAb is a very useful tool for clinical and experimental allergy studies. BMK-13 is a paneosinophil marker in both Frozen sections and Paraffin-Embedded tissues. It stains in bronchial sections 98% of the periphere blood eosinophils. The most useful for immunohistochemistry on frozen sections is the APAAP method. BMK-13 also binds to eosinophils in Paraffin-Embedded tissue. A 1:30 dilution in PBS is recommended. Incubation time: 1 hour at RT. Enzymatic pretreatment is necessary. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | No buffer |
| Preservative | 0.1% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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