![Western Blot: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] - Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) diluted at 1:500.](http://images.novusbio.com/fullsize/Enterovirus-71-VP1-Antibody-GT9512-Western-Blot-NBP3-13544-img0001.jpg "Western Blot: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] - Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) diluted at 1:500.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB |
| Clone | GT9512 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] - Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus 71 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) (1 ug/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-13544_mouse-enterovirus-71-vp1-mab-gt9512-910202420131277.jpg "ELISA: Enterovirus 71 VP1 Antibody (GT9512) [NBP3-13544] - Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus 71 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) (1 ug/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13544_mouse-enterovirus-71-vp1-mab-gt9512-western-blot-261120251820021.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (NBP3-13544) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
