DUX4 Antibody (E55)

Product Discontinued

DUX4 Antibody (E55) Summary

• Immunogen: Recombinant proteins, a mixture of two different protein fragments, of human DUX4 were used as an immunogen.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DUX4
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10 - 100
  • Western Blot 1:1000 - 10000

Reactivity Notes

Human, Mouse, Rat. Species cross-reactivity is based on Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for DUX4 Antibody (E55)

• double homeobox 4
• double homeobox protein 10
• double homeobox protein 4
• double homeobox protein 4/10
• double homeobox protein DUX10
• DUX10
• DUX4
• DUX4L1

Background

The DUX4 antibody, clone E5-5, was recently described in the publication called "Facioscapulohumeral dystrophy: incomplete suppression of a retrotransposed gene." (Snider L et al. PLoS Genet. 2010 Oct 28;6(10):e1001181).

DUX4, also known as double homeobox 4, contains two homeoboxes; the repeat-array and open reading frame (ORF) is conserved in other mammals. There was no evidence for transcription from standard cDNA libraries; however, RT-PCR and in-vitro expression experiments indicate that DUX4 has been reported to function as a transcriptional activator of paired-like homeodomain transcription factor 1. Mutations in DUX4 cause autosomal dominant facioscapulohumeral muscular dystrophy (FSHD) (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUX4 Antibody (NBP1-95878)(1)

Publication using NBP1-95878

• Snider L, Geng LN, Lemmers RJ et al. Facioscapulohumeral dystrophy: incomplete suppression of a retrotransposed gene. PLoS Genet 2010-10-01 [PMID: 21060811]

Bioinformatics

• Gene Symbol: DUX4
• Entrez:
  • 22947(Human)
• Uniprot:
  • Q9UBX2()