dsDNA Antibody (rDSD/4565)

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Immunocytochemistry/ Immunofluorescence: dsDNA Antibody (rDSD/4565) [NBP3-07670] - Immunofluorescent staining of PFA-fixed MCF-7 cells with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565) followed by goat ...read more
Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) [NBP3-07670] - Formalin-fixed, paraffin-embedded human colon stained with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565).
Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) [NBP3-07670] - Formalin-fixed, paraffin-embedded human lymph node stained with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC-P
Clone
rDSD/4565
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

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Catalog# & Formulation Size Price

dsDNA Antibody (rDSD/4565) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Nuclei of Burkitt's cells
Localization
Nuclear
Specificity
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This antibody recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This antibody produces a homogeneous staining pattern in the nucleus of normal and malignant cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Bioreactor concentrate
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Bioreactor concentrate

Notes

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08466)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for dsDNA Antibody (rDSD/4565)

  • Double stranded DNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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