DRAQ5™ - 0.5 mM Summary
Key Features of DRAQ5 (TM) :Bright, far-red DNA dye that works on live or fixed cell and tissue preparations for multiple applications such as flow cytometry, fluorescence imaging, and high content screening
Proven no-lyse, no-wash method for measuring DNA content in cells
Tested on hundreds of cell and tissue types
Exceptionally photostable so no photobleaching effect
Convenient, ready-to-use formulation
Enables phenotyping of blood/BM and direct cellular DNA content analysis in flow cytometry without any RBC lysis or fixation, permeabilization or RNase treatment
Spectrally compatible with most visible-range fluorophores including GFP/FITC and R-PE
- Flow Cytometry 1:25 - 1:100
- Fluorescence Imaging 1:100
- Immunocytochemistry/Immunofluorescence 1:100
- Live Imaging Microscopy 1:100
DRAQ5 (TM) is supplied at a concentration of 0.5 mM for 250 Imaging assays or 100 Flow Cytometry assays. It can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hankss, etc.) and mixed with fixatives such as formaldehyde.
Packaging, Storage & Formulations
Store at 4C in the dark. Do not freeze.
Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. DRAQ5 (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.
Alternate Names for DRAQ5 (TM) - 0.5 mM
Deep Red Anthraquinone 5 (DRAQ5 (TM)) is a cell-permeable dye belonging to the anthraquinone family that intercalates with high affinity to double stranded DNA (dsDNA). This far-red emitting fluorescent DNA dye is used for cell cycle analysis and nuclear staining. No measurable binding to mitochondrial DNA (mtDNA) has been detected and DRAQ5 (TM) shows negligible binding to RNA.
The excitation wavelength maxima of DRAQ5 (TM) are 600 nm / 646 nm but it can also be suboptimally excited by the 488nm (blue laser) for flow cytometry. Its emission wavelength maximum is 697 nm when intercalated with dsDNA. The fluorescence signal is proportional and stoichiometric to the quantity of nuclear DNA present in the cell.
DRAQ5 (TM) is a registered trademark of BioStatus Limited.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed
for 6 months from date of receipt.
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