DO-11.10 T cell Receptor Antibody (KJ1-26) [FITC]

Product Discontinued

DO-11.10 T cell Receptor Antibody (KJ1-26) [FITC] Summary

• Immunogen: The immunogen for this antibody is DO-11.10 T cell Receptor.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of the DO11.10 T cell hybridoma. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This KJ1-26 (KJ126, KJI-26, KJI26) antibody has also been reported for use in immunoprecipitation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for DO-11.10 T cell Receptor Antibody (KJ1-26) [FITC]

• DO 11.10
• DO-11.10
• KJ1.26

Background

The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of IAd major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.