DNA Antibody (SPM603)

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Immunohistochemistry-Paraffin: DNA Antibody (SPM603) [NBP2-45205] - Human Tonsil stained with Double Stranded DNA Monoclonal Antibody (SPM603)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC-P, ICC/IF
Clone
SPM603
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

DNA Antibody (SPM603) Summary

Immunogen
Nuclei of Burkitt's cells
Localization
Nuclear
Marker
Nuclear Marker
Specificity
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes; DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly associated pair of molecules in the shape of a right-handed double helix. Hydrogen bonds as well as forces generated by the hydrophobic effect and pi stacking hold the two DNA strands together. During replication and transcription, portions of the helix unwind and become single stranded. Protective proteins surround these single-stranded DNA. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Immunocytochemistry 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Immunocytochemistry Acetone-fixed cells 0.5 - 1.0 ug/ml for 30 minutes at RTImmunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for DNA Antibody (SPM603)

  • Double stranded deoxyribonucleic acid
  • Double stranded DNA
  • dsDNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NBP2-45205

Blogs on DNA.

World DNA Day Infographic
World DNA Day celebrates the anniversary of the discovery of the double helix and publication of the findings by Watson, Crick, Wilkins and Franklin as well as the completion of the Human Genome Project in 2003. Congress officially declared April 25th...  Read full blog post.

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