DNA Antibody (AC-30-10)

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Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Ch, ISpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
AC-30-10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

DNA Antibody (AC-30-10) Summary

Immunogen
Native DNA
Specificity
This reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10
  • Immunohistochemistry-Paraffin 1:10
Publications
Read Publications using NB110-89473.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline,0.5% Bovine Serum Albumin
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
IgM purified

Alternate Names for DNA Antibody (AC-30-10)

  • Double stranded deoxyribonucleic acid
  • Double stranded DNA
  • dsDNA

Background

dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Antibody (NB110-89473)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA Antibody (NB110-89473). (Showing 1 - 1 of 1 FAQs).

  1. Is the DNA antibody AC-30-10 specific against double-stranded DNA or does it also bind to single-stranded DNA?
    • According to our datasheet, NB110-89473 reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa. It should bind both DNA forms.

Secondary Antibodies

 

Isotype Controls

Additional DNA Products

DNA NB110-89473

Research Areas for DNA Antibody (NB110-89473)

Find related products by research area.

Blogs on DNA.

World DNA Day Infographic
World DNA Day celebrates the anniversary of the discovery of the double helix and publication of the findings by Watson, Crick, Wilkins and Franklin as well as the completion of the Human Genome Project in 2003. Congress officially declared April 25th...  Read full blog post.

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