Disialoganglioside GD3 Antibody (MB3.6) - BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P
Clone
MB3.6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Disialoganglioside GD3 Antibody (MB3.6) - BSA Free Summary

Immunogen
FM9 melanoma cells.
Specificity
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug per 10^6 cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (frozen tissue sections) Immunofluorescence of cells in culture. Flow cytometry: Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985). Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572. TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Phosphate Buffer (pH 7.6), 0.25 M NaCl
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Disialoganglioside GD3 Antibody (MB3.6) - BSA Free

  • 3g11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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