Digoxigenin Antibody (21H8) - Azide and BSA Free

• Reactivity: All
• Applications: WB, ELISA, Func, IHC
• Clone: 21H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Digoxigenin Antibody (21H8) - Azide and BSA Free Summary

• Immunogen: Digoxigenin
• Specificity: Reacts with free and bound digoxigenin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:10000
  • Immunohistochemistry 1:200-1:2000
  • In-situ Hybridization Reported in scientific literature (PMID:34954904)
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris , 0.1M Glycine and 2% Sucrose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Digoxigenin Antibody (21H8) - Azide and BSA Free

• DIG
• Digoxigenin

Background

Digoxigenin is a small organic molecule rather than amino acids. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Digoxigenin Antibody (NBP2-31191)(6)

Publications using NBP2-31191

• Chan TC, Chen YT, Tan KT et al. Biological significance of MYC and CEBPD coamplification in urothelial carcinoma: Multilayered genomic, transcriptional and posttranscriptional positive feedback loops enhance oncogenic glycolysis Clinical and translational medicine 2021-12-01 [PMID: 34954904] (ISH)
• Whelan DR, Lee WTC, Marks F et al. Super-resolution visualization of distinct stalled and broken replication fork structures PLoS Genet 2020-12-28 [PMID: 33370257]
• Kyle J Tomek, Kevin Volkel, Alexander Simpson, Austin G Hass, Elaine W Indermaur, James M Tuck, Albert J Keung Driving the Scalability of DNA-Based Information Storage Systems. ACS synthetic biology 2020-06-15 [PMID: 31117362]
• Babapoor S, Horwich M, Wu R et al. microRNA in situ hybridization for miR-211 detection as an ancillary test in melanoma diagnosis Mod. Pathol. 2016-02-26 [PMID: 26916074] (ISH)
  
  Details:
  This citation used the HRP version of this antibody.
• Babapoor S, Fleming E, Wu R, Dadras SS. A Novel miR-451a isomiR, Associated with Amelanotypic Phenotype, Acts as a Tumor Suppressor in Melanoma by Retarding Cell Migration and Invasion PLoS ONE 2014-09-20 [PMID: 25237911] (IF/IHC)
  
  Details:
  This citation used the HRP version of this antibody.
• Ronander E, Bengtsson DC, Joergensen L. Analysis of single-cell gene transcription by RNA fluorescent in situ hybridization (FISH) J Vis Exp 2012-10-01 [PMID: 23070076]
  
  Details:
  This citation used the HRP version of this antibody.