Dibromo-tyrosine Antibody (6G3)

Western Blot: Dibromo-tyrosine Antibody (6G3) [NBP2-59350] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3 (NBP2-59350). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (NBP2-59350) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.

Immunocytochemistry/Immunofluorescence: Dibromo-tyrosine Antibody (6G3) [NBP2-59350] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3 (NBP2-59350). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (NBP2-59350) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Immunohistochemistry: Dibromo-tyrosine Antibody (6G3) [NBP2-59350] - Immunohistochemistry analysis using Mouse Anti-Dibromotyrosine Monoclonal Antibody, Clone 6G3 (NBP2-59350). Tissue: Liver Cancer. Species: Human. Primary Antibody: Mouse Anti-Dibromotyrosine Monoclonal Antibody (NBP2-59350) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Western Blot: Dibromo-tyrosine Antibody (6G3) [NBP2-59350] - Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3 (NBP2-59350). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (NBP2-59350) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence: Dibromo-tyrosine Antibody (6G3) [NBP2-59350] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). Courtesy of: Dr. Robert Burke, University of Victoria.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Dibromo-tyrosine Antibody (6G3) Summary

• Immunogen: Synthetic 3,5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for 3,5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.