Dengue Virus Antibody (Dengue 1-11(3))

• Reactivity: V-Vi
• Applications: WB, ELISA, IHC, IHC-P
• Clone: Dengue 1-11(3)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Dengue Virus Antibody (Dengue 1-11(3)) Summary

• Immunogen: Made to Viral DENGUE VIRUS
• Specificity: NB100-65685 recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4 least strongly) of the genus Flavivirus. NB100-65685 detects an anti-E/envelope specific band of 61kDa under reducing conditions in Western blotting. A weak secondary band of 80kDa may also be apparent.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Dengue Virus Antibody (Dengue 1-11(3))

• DEN 1
• DEN1
• Dengue hemorrhagic fever
• Dengue type 1 virus
• DENV 1
• DENV1

Background

Dengue (DF) and dengue hemorrhagic fever (DHF) are caused by one of four closely related, but antigenically distinct, virus serotypes (DEN-1, DEN-2, DEN-3, and DEN-4), of the genus Flavivirus. Infection with one of these serotypes provides immunity to only that serotype for life, so persons living in a dengue-endemic area can have more than one dengue infection during their lifetime. DF and DHF are primarily diseases of tropical and sub tropical areas, and the four different dengue serotypes are maintained in a cycle that involves humans and the Aedes mosquito.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Dengue Virus Antibody (NB100-65685). (Showing 1 - 1 of 1 FAQ).

1. I would like to set up immunoassay for the detection of Dengue virus in tissue culture media from infected cells. The product sheet for anti-Dengue Virus, NB100-65685, states that this antibody can be used in an ELISA. Could you provide more details regarding this application?
   • The antibody was tested for indirect ELISA using a dilution range of 1 to 10ug/ml. The virions were coated directly on to the plate using standard coating procedures. The secondary antibody was a goat anti mouse IgG (HRP conjugated).