Decapentaplegic/DPP Antibody [Unconjugated]

• Reactivity: Dr
• Applications: WB
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: LYOPH

Decapentaplegic/DPP Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant Drosophila Decapentaplegic/DPP
  Asp457-Arg588 (Gln473His, Pro474Ala)
  Accession # P07713
• Specificity: Detects Drosophila Decapentaplegic/DPP in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) TGF-beta 1, rhTGF-beta 2, rhTGF-beta 3, and recombinant amphibian TGF‑ beta 5 is observed.
• Source: N/A
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Purity Statement: Antigen Affinity-purified from egg yolks

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decapentaplegic/DPP Antibody [Unconjugated]

• Decapentaplegic
• DPP

Background

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional orthologue of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks. Similar to other TGF-beta family ligands, Dpp is synthesized as a large proprotein which is proteolytically processed at the dibasic cleavage site to release the carboxy-terminal domain. Biologically active Dpp is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot and the interchain disulfide bond. Cellular responses to Dpp have been shown to be mediated by the ligand-induced formation of heteromeric complexes of the Drosophila type I, Thick Veins (Tkv), and type II, Punt, serine/threonine kinases. The activated receptor complex induces the phosphorylation of the prototypical Smad, Mad, and subsequent translocation of the Mad‑Medea complex to the nucleus where they regulate the transcription of target genes. Secreted extracellular Dpp antagonists, including the short-gastrulation (Sog) and twisted gastrulation (TSG), which bind Dpp and regulate its availability, have been identified.

1. Raftery, L.A. and D.J. Sutherland (1999) Dev. Biol. 210:251.
2. Ruberte, E. et al. (1995) Cell 80:890.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Decapentaplegic/DPP Antibody (AF159)(1)

Publication using AF159

• Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila. Dev. Cell, 2008-06-01;14(6):940-53. 2008-06-01 [PMID: 18539121] (Western Blot, Drosophila)

Bioinformatics

• Uniprot:
  • P07713()