Cytokinesis Antibody Pack

Western Blot: Cytokinesis Antibody Pack 3vials [NBP1-78943] - Western blot analysis of Borealin on mouse kidney lysate using NBP1-22832.

Immunocytochemistry/Immunofluorescence: Cytokinesis Antibody Pack [NBP1-78943] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Western Blot: Cytokinesis Antibody Pack 3vials [NBP1-78943] - Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) using NB110-92717.

Product Discontinued

Cytokinesis Antibody Pack Summary

• Description: This pack contains 1 vial each of: NB110-92717 (25 uL), NBP1-22832 (25 uL), NB100-353 (25 uL).
• Immunogen: See individual datasheets.
• Specificity: This Cytokinesis Antibodies Sample Pack includes antibodies to Borealin, BubR1 and Survivin.

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Mainly Western blot, however additional applications may be possible for individual products. See individual datasheets for validated applications.

Reactivity Notes

Human, however additional species may be possible for individual products.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: See individual datasheets.

Background

In cytokinesis, survivin is a critical part of spindle maintenance. For cytokinesis to occur, Borealin and BubR1 need to interact with Survivin. Spindle analysis in mitosis can be optimized by simultaneous analysis of all three proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.