![Immunohistochemistry-Paraffin: Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115) - IHC-Prediluted [NBP2-48219] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin, LMW Monoclonal Antibody (SPM115)](http://images.novusbio.com/fullsize/Cytokeratin-Acidic-Type-I-or-LMW-Antibody-SPM115-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48219-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115) - IHC-Prediluted [NBP2-48219] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin, LMW Monoclonal Antibody (SPM115)")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, ReSpecies Glossary |
| Applications | IHC |
| Clone | SPM115 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Human epidermal keratin (Uniprot: Q7Z794) |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. monoclonal antibody SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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