Cre Antibody

Western Blot: Cre Antibody [NBP1-47646] - Analysis of Cre at 1:2000. Lane 1, HeLa cells stably transfected with a CRE expression plasmid. Lane 2, HeLa cells transfected with a neo plasmid (negative control).

Immunohistochemistry-Paraffin: Cre Antibody [NBP1-47646] - Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Cre Antibody [NBP1-47646] - Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in brain neurons . This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.

Product Discontinued

Cre Antibody Summary

• Immunogen: Synthethic peptide corresponding to amino acids 57-73 (KLNNRKWFPAEPEDVRD) of the bacteriophage protein CRE.
• Specificity: CRE is detected at ~36-38 kDa on western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Cre Antibody

• Cyclization recombinase

Background

The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cre Antibody (NBP1-47646)(2)

Publications using NBP1-47646

• Copple BL, Kaska S, Wentling C. Hypoxia-inducible factor activation in myeloid cells contributes to the development of liver fibrosis in cholestatic mice. J Pharmacol Exp Ther. 2012-05-01 [PMID: 22271822] (IHC-Fr, Mouse)
  
  Details:
  IHC (frozen): Fig 1B, D (mouse liver). The Cre antibody (IMG-5720. IMG-5721 or IMG-5722) was used at 1:4000.
• Banares S, Zeh K, Krajewska M et al. Novel pan-neuronal Cre-transgenic line for conditional ablation of genes in the nervous system. Genesis. 2005-05-01 [PMID: 15828007] (WB)
  
  Details:
  1. IMG-5720 (AR-59A): WB., IHC-P. 2. IMG-5721 (AR-59B): WB, IHC-F. 3. IMG-5722 (AR-60A): IHC-P

FAQs for Cre Antibody (NBP1-47646). (Showing 1 - 1 of 1 FAQs).

1. A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
   • With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.