Cre Antibody

Images

 
Western Blot: Cre Antibody [NBP1-47646] - Analysis of Cre at 1:2000. Lane 1, HeLa cells stably transfected with a CRE expression plasmid. Lane 2, HeLa cells transfected with a neo plasmid (negative control).
Immunohistochemistry-Paraffin: Cre Antibody [NBP1-47646] - Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse at 1:2000. In this ...read more
Immunohistochemistry-Paraffin: Cre Antibody [NBP1-47646] - Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse at 1:2000. In this mouse ...read more

Product Details

Summary
Reactivity CsSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cre Antibody Summary

Immunogen
Synthethic peptide corresponding to amino acids 57-73 (KLNNRKWFPAEPEDVRD) of the bacteriophage protein CRE.
Specificity
CRE is detected at ~36-38 kDa on western blots.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
This antibody is useful for immunohistochemistry-Frozen, immunohistochemistry-Paraffin, immunoprecipitation and Western blot.
Publications
Read Publications using
NBP1-47646 in the following applications:

Reactivity Notes

Cre/lox Systems Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Background

The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for Cre Antibody (NBP1-47646)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cre Antibody (NBP1-47646). (Showing 1 - 1 of 1 FAQs).

  1. A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
    • With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

Secondary Antibodies

 

Isotype Controls

Additional Cre Products

Cre NBP1-47646

Blogs on Cre.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

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