COS-7 Cell Lysate

Product Discontinued

COS-7 Cell Lysate Summary

• Description: Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml and mixed with 5x SDS sample buffer to become 1xSDS sample buffer (2% SDS, 300 mM beta-mercaptoethanol, 50 mM Tris-HCl pH 6.8, 10 % Glycerol, & 0.01% Bromophenol blue) at final 3 mg/ml. The lysate was heated at 95 degreesC for 5 min, and cooled rapidly.
• Specificity: Tissue: Kidney; SV40 transformed

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Lysate Details for Array

• Type: Cell

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

The COS cell line is derived from kidney cells of African Green monkeys. COS-7 is a form of the COS cell line that is often used in studies of the simian virus SV40, which has been linked to some varieties of human cancer. Some COS cells have been shown to constitutively express the potentially oncogenic protein, T-antigen. COS-7 cells are also used to create recombinant proteins for biological applications, especially transfection experiments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.