Cortisol Antibody (CORT-1) - BSA Free

• Reactivity: Hu
• Applications: ELISA
• Clone: CORT-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Cortisol Antibody (CORT-1) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified cortisol conjugated with BSA.
• Localization: Secreted.
• Specificity: Cortisol-BSA conjugate and free cortisol. There is no cross-reactivity with BSA. Cross-reactivity, % 50% displacement of cortisol-3-125-Iodine: Cortisol (100)%; Corticosterone (20%); 17-Hydroxyprogesterone (0%)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
• Application Notes: Detection of cortisol in competitive assay.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Cortisol Antibody (CORT-1) - BSA Free

• Compound F
• Cortisol
• Hydrocortisone

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cortisol Antibody (NB200-428). (Showing 1 - 1 of 1 FAQ).

1. Has your Cortisol monoclonal antibody (NB200-428) been tested for cross-reactivity against the following list of compounds? Corticosterone, Prednisone, Progesterone, Cortisone, 11-deoxycortisol, Prednisolone, Testosterone, Other.
   • We have tested NB200-428 against the following: 11-Deoxycortisol (N/A), Prednisolone (N/A), Corticosterone (20%), 11-Deoxycorticosterone (N/A), Progesterone (N/A), 17-Hydroxyprogesterone (0%), Testosterone, Estradiol, Estriol (N/A), Danazol (N/A).